human il-2 elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: Frontiers in Immunology

Article Title: VISTA + follicular regulatory T cells modulate the function of effector immune cells: implications for ovarian cancer immune escape

doi: 10.3389/fimmu.2025.1704048

Figure Lengend Snippet: Effects of VISTA + /VISTA − Tfr cells on CD8 + T cell proliferation and cytokine secretion. After co-culture of VISTA + or VISTA − Tfr cells with CD8 + T cells (CD8 + T cells: Tfr cells = 4:1): (A) Representative flow cytometry histogram of CFSE proliferation assay for co-cultured cells. The x-axis represents CFSE fluorescence intensity, and the y-axis represents cell count (n=3). (B) Quantitative analysis of CFSE fluorescence intensity in VISTA − Tfr + CD8 + T cell group versus VISTA + Tfr + CD8 + T cell group. (C) Quantitative analysis of CFSE fluorescence intensity after co-culture of CD8 + T cells with VISTA − Tfr cells overexpressing VISTA (LV-VISTA). (D) Quantitative analysis of CFSE fluorescence intensity after co-culture of CD8 + T cells with VISTA + Tfr cells with VISTA silenced (VISTA-shRNA). (E–L) Levels of IL-2, IFN-γ, perforin (PFP), and granzyme B in culture supernatants of VISTA − Tfr + CD8 + T cell group (E–H) and VISTA + Tfr + CD8 + T cell group (I–L) as detected by ELISA (n=3). The statistical results are expressed as the mean ± SD, * p < 0.05, ** p < 0.01.

Article Snippet: For co-culture groups with CD8 + T lymphocytes, levels of IL-2, Interferon-γ (IFN-γ), granzyme B, and perforin were detected (MultiSciences, EK102, EK180, EK158; CUSABIO, CSB-E09313h).

Techniques: Co-Culture Assay, Flow Cytometry, Proliferation Assay, Cell Culture, Fluorescence, Cell Counting, shRNA, Enzyme-linked Immunosorbent Assay